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pure line 【生物學】純系。

purely

These results demonstrated that merely examining the segregation of reporter gene in pollens is a reliable method that could avoid the laborious testing process of the pure line from transformed plants ; ( 3 ) the transcript levels of leetrl and leetrl were checked by northern blotting analysis ( 3 )兩個基因在反義植株里的表達都受到不同程度的抑制。在反義leetr1植株里, leetr1表達被抑制后, leetr2的表達也受到不同程度的抑制。同時,反義leetr2植株里也有相似的結果。

Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions 本研究以反義乙烯受體leetr1 , leetr2基因番茄t _ 0代種子為實驗材料,利用gus基因表達研究外源基因的遺傳規律,并借助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因后代的生長發育模式、對外源乙烯敏感性,以及靶基因的表達特性,初步探明了它們在乙烯受體系統中的功能。

It is differ both from the method of “ circulate line selection “ for seed reproduction and from the method of “ pure line breeding “ for varieties development 該方法既區別于單純生產原種的“株系循環法” ,也區別于選育新品種的純系育種法。

Selection of somatic embryogenesis pure lines of two upland cotton gossypium hirsutum l . cultivars 兩個陸地棉體細胞胚胎發生新品系的選育

When a pure line variety is grown at close spacing there is a competition effect . 當一個純系品種在密的植距下生長時有一種競爭效應。